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231223s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2010.06.012
|2 doi
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|a pubmed24n0666.xml
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|a (DE-627)NLM199804761
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|a (NLM)20655621
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Rosales-Mendoza, Sergio
|e verfasserin
|4 aut
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|a Transgenic carrot tap roots expressing an immunogenic F1-V fusion protein from Yersinia pestis are immunogenic in mice
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 07.04.2011
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2010 Elsevier GmbH. All rights reserved.
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|a Expression of the protective F1 and V antigens of Yersinia pestis, as a fusion protein, in carrot was pursued in an effort to develop an alternative vaccine production system against the serious plague disease. Transgenic carrot plants carrying the F1-V encoding gene were developed via Agrobacterium-mediated transformation. Presence, integration, and expression of the F1-V encoding gene were confirmed by polymerase chain reaction (PCR), DNA gel blot analysis, and reverse-transcriptase (RT)-PCR analyses, respectively. An ELISA assay confirmed the antigenicity of the plant-derived F1-V fusion protein. Immunogenicity was evaluated subcutaneously in mice using a soluble protein extract of freeze-dried transgenic carrot. Significant antibody levels were detected following immunization. These results demonstrated that the F1-V protein could be expressed in carrot tap roots, and that the carrot F1-V recombinant protein retained its antigenicity and immunogenicity
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antigens, Bacterial
|2 NLM
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| 650 |
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|a Bacterial Proteins
|2 NLM
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| 650 |
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|a LcrV protein, Yersinia
|2 NLM
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| 650 |
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|a Plague Vaccine
|2 NLM
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| 650 |
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|a Pore Forming Cytotoxic Proteins
|2 NLM
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| 650 |
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|a Recombinant Fusion Proteins
|2 NLM
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| 650 |
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|a caf1 protein, Yersinia pestis
|2 NLM
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| 700 |
1 |
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|a Soria-Guerra, Ruth E
|e verfasserin
|4 aut
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1 |
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|a Moreno-Fierros, Leticia
|e verfasserin
|4 aut
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| 700 |
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|a Han, Yuepeng
|e verfasserin
|4 aut
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| 700 |
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|a Alpuche-Solís, Angel G
|e verfasserin
|4 aut
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| 700 |
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|a Korban, Schuyler S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 2 vom: 15. Jan., Seite 174-80
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:2
|g day:15
|g month:01
|g pages:174-80
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|u http://dx.doi.org/10.1016/j.jplph.2010.06.012
|3 Volltext
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