Attenuation of invariant natural killer T-cell anergy induction through intradermal delivery of alpha-galactosylceramide

Attenuation of invariant natural killer T-cell anergy induction through intradermal delivery of alpha-galactosylceramide

Copyright 2010 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 136(2010), 3 vom: 15. Sept., Seite 364-74
1. Verfasser: Bontkes, Hetty J (VerfasserIn)
Weitere Verfasser: Moreno, María, Hangalapura, Basav, Lindenberg, Jelle J, de Groot, Jan, Lougheed, Sinéad, van der Vliet, Hans J J, van den Eertwegh, Alfons J M, de Gruijl, Tanja D, von Blomberg, B Mary E, Scheper, Rik J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Adjuvants, Immunologic Cytokines Galactosylceramides alpha-galactosylceramide Ovalbumin 9006-59-1
Beschreibung
Zusammenfassung:Copyright 2010 Elsevier Inc. All rights reserved.
CD1d restricted, alpha-galactosylceramide (alphaGC) responsive invariant (i)NKT cells positively regulate immune responses. Both intravenous and intradermal administered alphaGC are known to activate iNKT cells. iNKT cells become unresponsive to a second intravenous alphaGC injection, whereas no data are available regarding potential anergy upon intradermal administration. Here, comparative analysis of two intradermal versus two intravenous injections in mice demonstrated that iNKT cell anergy was prevented by intradermal injection and when combined with a vaccine, superior tumor protection afforded by intradermally administered alphaGC. Moreover, human skin dendritic cells (DC) took up intradermally injected alphaGC and activated iNKT cells upon migration, while iNKT cells in human skin-draining lymph nodes expanded in response to alphaGC presented either by exogenously added DC or by CD1d positive antigen presenting cells in the lymph nodes. In conclusion, glycolipids such as alphaGC may greatly improve the efficacy of skin immunization strategies, targeting cutaneous and lymph node DC
Beschreibung:Date Completed 30.08.2010
Date Revised 06.08.2010
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2010.04.019