Normalization of genomic DNA using duplex-specific nuclease
An application of duplex-specific nuclease (DSN) normalization technology to whole-genome shotgun sequencing of genomes with a large proportion of repetitive DNA is described. The method uses a thermostable DSN from the Kamchatka crab that specifically hydrolyzes dsDNA. In model experiments on human...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 48(2010), 6 vom: 01. Juni, Seite 455-9
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| 1. Verfasser: |
Shagina, Irina
(VerfasserIn) |
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| Weitere Verfasser: |
Bogdanova, Ekaterina,
Mamedov, Ilgar Z,
Lebedev, Yury,
Lukyanov, Sergey,
Shagin, Dmitry |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA
9007-49-2
Deoxyribonucleases
EC 3.1.- |
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