Protein mediated miRNA detection and siRNA enrichment using p19
p19 RNA binding protein from the Carnation Italian ringspot virus (CIRV) is an RNA-silencing suppressor that binds small interfering RNA (siRNA) with high affinity. We created a bifunctional p19 fusion protein with an N-terminal maltose binding protein (MBP), for protein purification, and a C-termin...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1988. - 48(2010), 6 vom: 01. Juni, Seite xvii-xxiii
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| 1. Verfasser: |
Jin, Jingmin
(VerfasserIn) |
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| Weitere Verfasser: |
Cid, Melissa,
Poole, Catherine B,
McReynolds, Larry A |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Maltose-Binding Proteins
MicroRNAs
P19 protein, tomato bushy stunt virus
Periplasmic Binding Proteins
RNA, Small Interfering
RNA-Binding Proteins
Recombinant Fusion Proteins
Viral Proteins |
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