Application of HaloTag protein to covalent immobilization of recombinant proteins for single molecule force spectroscopy
We have developed the HaloTag system for the covalent immobilization of polyproteins onto a mica substrate for single molecule force spectroscopy using the atomic force microscope. A recombinant fusion polyprotein of titin I27 with HaloTag7 protein was produced, and the covalent and site-specific at...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 26(2010), 13 vom: 06. Juli, Seite 10433-6 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Recombinant Fusion Proteins Hydrolases EC 3.- haloalkane dehalogenase EC 3.8.1.5 |
| Online verfügbar |
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