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|a pubmed25n0662.xml
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|a (DE-627)NLM198546564
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|a (NLM)20519083
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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1 |
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|a Wang, Hua-jun
|e verfasserin
|4 aut
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|a The anti-apoptosis effect of erythropoietin on neonatal rat cardiocytes during hypoxia/reoxygenation injury and its possible mechanism
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 08.07.2011
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|a Date Revised 03.06.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To investigate the anti-apoptosis effect of erythropoietin (EPO) on myocardial cells after hypoxia/reoxygenation in vitro, and the relationship among protein kinase C (PKC), the mitochondrial ATP-sensitive potassium (mitoKATP) channel and EPO in the anti-apoptotic signaling pathways
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|a METHODS: Cardiocytes were harvested from neonatal rats and cultured. Cultured myocardial cells were divided into the control group, the hypoxia/reoxygenation group, the EPO group and the chelerythrine group, and a hypoxia/reoxygenation model of cardiocytes was reproduced. Apoptosis rate was assayed by flow cytometry. Flavoprotein fluorescence was scanned by confocal laser microscope to assess the mitoKATP channel activity
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|a RESULTS: Apoptosis rate was significantly higher in hypoxia/reoxygenation group than that of control group [(42.56+/-8.00)% vs. (17.88+/-2.00)%, P<0.05]. There was no statistically significant difference in flavoprotein fluorescence between this group and the control group [(0.278+/-0.170)x10(-2) vs. (0.149+/-0.050)x10(-2), P>0.05]. Myocardial cell apoptosis rate in EPO group was lower than that in hypoxia/reoxygenation group [(22.73+/-5.00)% vs. (42.56+/-8.00)%, P<0.05], and flavoprotein fluorescence intensity was significantly enhanced when compared with hypoxia/reoxygenation group [(2.201+/-1.090)x10(-2) vs. (0.278+/-0.170)x10(-2), P<0.01]. However, when chelerythrine was added, the anti-apoptosis effect of EPO was blocked, and the intensity of cardiocytes flavoprotein fluorescence was decreased [the apoptosis rate was (46.72+/-17.00)% and the flavoprotein fluorescence intensity was (0.986+/-0.320)x10(-2) ]. When compared with EPO group there was statistically significant difference (P<0.01 and P<0.05)
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|a CONCLUSION: Myocardial cell apoptosis occurs in hypoxia/reoxygenation injury, and EPO can protect rat cardiomyocytes from hypoxia/reoxygenation induced apoptosis. The protective effect is partly associated with the PKC/mitoKATP pathway
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Potassium Channels
|2 NLM
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|a mitochondrial K(ATP) channel
|2 NLM
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|a Erythropoietin
|2 NLM
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| 650 |
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|a 11096-26-7
|2 NLM
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| 650 |
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|a Protein Kinase C
|2 NLM
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| 650 |
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|a EC 2.7.11.13
|2 NLM
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| 700 |
1 |
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|a Jiang, Hui-lin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Xiao-hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lin, Pei-yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhu, Yong-cheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tao, Li-li
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 22(2010), 5 vom: 27. Mai, Seite 302-5
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:22
|g year:2010
|g number:5
|g day:27
|g month:05
|g pages:302-5
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_ILN_350
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|a AR
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|d 22
|j 2010
|e 5
|b 27
|c 05
|h 302-5
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