Effects of charge double layer and colloidal aggregation on the isotropic-nematic transition of protein fibers in water
We investigate the effects of variable linear charge density and Debye length on the mesoscopic properties of beta-lactoglobulin fibers in water, by changing the pH and ionic strength, respectively. We determine the isotropic-nematic (I-N) transition by cross-polarized microscopy and quantify by ato...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 13 vom: 06. Juli, Seite 10401-5 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Lactoglobulins Water 059QF0KO0R |
| Zusammenfassung: | We investigate the effects of variable linear charge density and Debye length on the mesoscopic properties of beta-lactoglobulin fibers in water, by changing the pH and ionic strength, respectively. We determine the isotropic-nematic (I-N) transition by cross-polarized microscopy and quantify by atomic force microscopy the increasing tendency of the fibers to aggregate upon raising ionic strength. We then compare experimental I-N transitions with theoretical expected values based on Onsager theory. Unlike previous reports on lyotropic liquid crystalline behavior of protein fibers, we show that, if double layer effects and aggregation of fibers are correctly included directly in the second virial coefficient and excluded volume, Onsager theory accurately predicts the experimental I-N transition versus pH and ionic strength |
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| Beschreibung: | Date Completed 30.09.2010 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la101636r |