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20250211142439.0 |
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cr uuu---uuuuu |
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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1016/j.clim.2010.04.008
|2 doi
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|a pubmed25n0661.xml
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|a (NLM)20493777
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zelini, Paola
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Human cytomegalovirus-specific CD4(+) and CD8(+) T-cell response determination
|b comparison of short-term (24h) assays vs long-term (7-day) infected dendritic cell assay in the immunocompetent and the immunocompromised host
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| 264 |
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|c 2010
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 05.08.2010
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| 500 |
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|a Date Revised 12.07.2010
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Human cytomegalovirus (HCMV)-specific CD4(+) and CD8(+) T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV-infected dendritic cells (iDC), and two short-term (24h) assays, one for CD4(+) stimulation by infected cell lysate (iCL), and the other for CD8(+) stimulation by a pool of 34 epitopic peptides (pep-pool). In the immunocompetent, the number of T-cells activated by either iCL or the pep-pool was significantly reduced with respect to iDC. In both SOTR and HSCTR, the number of T-cells activated by iDC was comparable to that activated by iCL or the pep-pool. A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay
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| 650 |
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|a Comparative Study
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 700 |
1 |
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|a Lilleri, Daniele
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Comolli, Giuditta
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rognoni, Vanina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chiesa, Antonella
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fornara, Chiara
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Locatelli, Franco
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Meloni, Federica
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gerna, Giuseppe
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 136(2010), 2 vom: 01. Aug., Seite 269-81
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
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| 773 |
1 |
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|g volume:136
|g year:2010
|g number:2
|g day:01
|g month:08
|g pages:269-81
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| 856 |
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|u http://dx.doi.org/10.1016/j.clim.2010.04.008
|3 Volltext
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|d 136
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|h 269-81
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