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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la100344f
|2 doi
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|a pubmed24n0661.xml
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|a (NLM)20486681
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|a DE-627
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|e rakwb
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|a eng
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|a Tang, S C
|e verfasserin
|4 aut
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|a UV-induced grafting processes with in situ formed photomask for micropatterning of two-component biomolecules
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 31.08.2010
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive UV-initiated grafting processes, under the assistance of the in situ formed poly (4,4'-bi[N-(4-vinylbenzyl) pyridinium]) (P(BVV)) photomask. The epoxy groups of the P(GMA) microdomains can be aminated for covalently coupling biotin, while the P(AA) microdomains were used for immobilizing immunoglobulin (IgG). The resulting biotin- and IgG-coupled microdomains interact specifically with their corresponding target proteins, avidin and anti-IgG, respectively
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|a Journal Article
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|a Antibodies, Anti-Idiotypic
|2 NLM
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|a Immunoglobulin G
|2 NLM
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|a Polymers
|2 NLM
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|a Avidin
|2 NLM
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|a 1405-69-2
|2 NLM
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|a Biotin
|2 NLM
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|a 6SO6U10H04
|2 NLM
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|a Xie, J Y
|e verfasserin
|4 aut
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|a Huang, Z H
|e verfasserin
|4 aut
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|a Xu, F J
|e verfasserin
|4 aut
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|a Yang, Wantai
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 12 vom: 15. Juni, Seite 9905-10
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:26
|g year:2010
|g number:12
|g day:15
|g month:06
|g pages:9905-10
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|u http://dx.doi.org/10.1021/la100344f
|3 Volltext
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