UV-induced grafting processes with in situ formed photomask for micropatterning of two-component biomolecules

We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 12 vom: 15. Juni, Seite 9905-10
1. Verfasser: Tang, S C (VerfasserIn)
Weitere Verfasser: Xie, J Y, Huang, Z H, Xu, F J, Yang, Wantai
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Antibodies, Anti-Idiotypic Immunoglobulin G Polymers Avidin 1405-69-2 Biotin 6SO6U10H04
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520 |a We report a photolithographic process for micropatterning of two-component biomolecules on a transparent organic film via lateral functional polymer brushes of poly(sodium acrylate) (P(AA)) and poly(glycidyl methacrylate) (P(GMA)). The pattern of binary polymer brushes were prepared via consecutive UV-initiated grafting processes, under the assistance of the in situ formed poly (4,4'-bi[N-(4-vinylbenzyl) pyridinium]) (P(BVV)) photomask. The epoxy groups of the P(GMA) microdomains can be aminated for covalently coupling biotin, while the P(AA) microdomains were used for immobilizing immunoglobulin (IgG). The resulting biotin- and IgG-coupled microdomains interact specifically with their corresponding target proteins, avidin and anti-IgG, respectively 
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700 1 |a Xie, J Y  |e verfasserin  |4 aut 
700 1 |a Huang, Z H  |e verfasserin  |4 aut 
700 1 |a Xu, F J  |e verfasserin  |4 aut 
700 1 |a Yang, Wantai  |e verfasserin  |4 aut 
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