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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2010.03.007
|2 doi
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|a pubmed24n0660.xml
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|a (DE-627)NLM198017871
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|a (NLM)20462765
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Kittur, Farooqahmed S
|e verfasserin
|4 aut
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|a Deletion of the N-terminal dirigent domain in maize beta-glucosidase aggregating factor and its homolog sorghum lectin dramatically alters the sugar-specificities of their lectin domains
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.11.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright 2010 Elsevier Masson SAS. All rights reserved.
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|a Maize beta-glucosidase aggregating factor (BGAF) and its homolog Sorghum Lectin (SL) are modular proteins consisting of an N-terminal dirigent domain and a C-terminal jacalin-related lectin (JRL) domain. BGAF is a polyspecific lectin with a monosaccharide preference for galactose, whereas SL displays preference for GalNAc. Here, we report that deletion of the N-terminal dirigent domain in the above lectins dramatically changes their sugar-specificities. Deletions in the N-terminal region of the dirigent domain of BGAF abolished binding to galactose/lactose, but binding to mannose was unaffected. Glucose, which was a poor inhibitor of hemagglutinating activity of BGAF, displayed higher inhibitory effect on the hemagglutinating activity of deletion mutants. Deletion of the dirigent domain in SL abolished binding to GalNAc, but binding to mannose was not affected. Surprisingly, fructose, an extremely poor inhibitor (minimum inhibitory concentration (MIC) = 125 mM) of SL hemagglutinating activity, was found to be a very potent inhibitor (MIC = 1 mM) of hemagglutinating activity of its JRL domain. These results indicate that the dirigent domain in this class of modular lectins, at least in the case of maize BGAF and SL, influences sugar specificity
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a BGAF protein, Zea mays
|2 NLM
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| 650 |
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|a Carrier Proteins
|2 NLM
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| 650 |
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|a Lectins
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Fructose
|2 NLM
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|a 30237-26-4
|2 NLM
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| 650 |
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|a Glucose
|2 NLM
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| 650 |
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|a IY9XDZ35W2
|2 NLM
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| 650 |
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|a Lactose
|2 NLM
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| 650 |
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7 |
|a J2B2A4N98G
|2 NLM
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| 650 |
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|a Acetylgalactosamine
|2 NLM
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| 650 |
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|a KM15WK8O5T
|2 NLM
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| 650 |
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|a Mannose
|2 NLM
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| 650 |
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|a PHA4727WTP
|2 NLM
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| 650 |
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|a Galactose
|2 NLM
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| 650 |
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|a X2RN3Q8DNE
|2 NLM
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| 700 |
1 |
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|a Yu, Hyun Young
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bevan, David R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Esen, Asim
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 48(2010), 8 vom: 01. Aug., Seite 731-4
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
8 |
|g volume:48
|g year:2010
|g number:8
|g day:01
|g month:08
|g pages:731-4
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2010.03.007
|3 Volltext
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|a AR
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|d 48
|j 2010
|e 8
|b 01
|c 08
|h 731-4
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