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231223s2010 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la100879p
|2 doi
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|a pubmed24n0659.xml
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|a DE-627
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Hong, Jennifer S
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Microfluidic directed self-assembly of liposome-hydrogel hybrid nanoparticles
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| 264 |
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|c 2010
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 30.09.2010
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|a Date Revised 16.11.2017
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|a published: Print
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|a Citation Status MEDLINE
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|a We present a microfluidic method to direct the self-assembly of temperature-sensitive liposome-hydrogel hybrid nanoparticles. Our approach yields nanoparticles with structural properties and highly monodisperse size distributions precisely controlled across a broad range relevant to the targeted delivery and controlled release of encapsulated therapeutic agents. We used microfluidic hydrodynamic focusing to control the convective-diffusive mixing of two miscible nanoparticle precursor solutions (a DPPC:cholesterol:DCP phospholipid formulation in isopropanol and a photopolymerizable N-isopropylacrylamide mixture in aqueous buffer) to form nanoscale lipid vesicles with encapsulated hydrogel precursors. These precursor nanoparticles were collected off-chip and were irradiated with ultraviolet (UV) light in bulk to polymerize the nanoparticle interiors into hydrogel cores. Multiangle laser light scattering in conjunction with asymmetric flow field-flow fractionation was used to characterize nanoparticle size distributions, which spanned the approximately 150 to approximately 300 nm diameter range as controlled by microfluidic mixing conditions, with a polydispersity of approximately 3% to approximately 5% (relative standard deviation). Transmission electron microscopy was then used to confirm the spherical shape and core-shell composition of the hybrid nanoparticles. This method may be extended to the directed self-assembly of other similar cross-linked hybrid nanoparticle systems with engineered size/structure-function relationships for practical use in healthcare and life science applications
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Liposomes
|2 NLM
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| 650 |
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|a Hydrogel, Polyethylene Glycol Dimethacrylate
|2 NLM
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| 650 |
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|a 25852-47-5
|2 NLM
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| 700 |
1 |
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|a Stavis, Samuel M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a DePaoli Lacerda, Silvia H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Locascio, Laurie E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Raghavan, Srinivasa R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gaitan, Michael
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 13 vom: 06. Juli, Seite 11581-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:26
|g year:2010
|g number:13
|g day:06
|g month:07
|g pages:11581-8
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|u http://dx.doi.org/10.1021/la100879p
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