|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM197522165 |
| 003 |
DE-627 |
| 005 |
20231223210733.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231223s2010 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1093/jxb/erq114
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0658.xml
|
| 035 |
|
|
|a (DE-627)NLM197522165
|
| 035 |
|
|
|a (NLM)20410319
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Monreal, José Antonio
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinase
|
| 264 |
|
1 |
|c 2010
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.09.2010
|
| 500 |
|
|
|a Date Revised 03.12.2021
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a The photosynthetic phosphoenolpyruvate carboxylase (C(4)-PEPC) is regulated by phosphorylation by a phosphoenolpyruvate carboxylase kinase (PEPC-k). In Digitaria sanguinalis mesophyll protoplasts, this light-mediated transduction cascade principally requires a phosphoinositide-specific phospholipase C (PI-PLC) and a Ca(2+)-dependent step. The present study investigates the cascade components at the higher integrated level of Sorghum bicolor leaf discs and leaves. PEPC-k up-regulation required light and photosynthetic electron transport. However, the PI-PLC inhibitor U-73122 and inhibitors of calcium release from intracellular stores only partially blocked this process. Analysis of [(32)P]phosphate-labelled phospholipids showed a light-dependent increase in phospholipase D (PLD) activity. Treatment of leaf discs with n-butanol, which decreases the formation of phosphatidic acid (PA) by PLD, led to the partial inhibition of the C(4)-PEPC phosphorylation, suggesting the participation of PLD/PA in the signalling cascade. PPCK1 gene expression was strictly light-dependent. Addition of neomycin or n-butanol decreased, and a combination of both inhibitors markedly reduced PPCK1 expression and the concomitant rise in PEPC-k activity. The calcium/calmodulin antagonist W7 blocked the light-dependent up-regulation of PEPC-k, pointing to a Ca(2+)-dependent protein kinase (CDPK) integrating both second messengers, calcium and PA, which were shown to increase the activity of sorghum CDPK
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Enzyme Inhibitors
|2 NLM
|
| 650 |
|
7 |
|a Phosphatidic Acids
|2 NLM
|
| 650 |
|
7 |
|a 1-Butanol
|2 NLM
|
| 650 |
|
7 |
|a 8PJ61P6TS3
|2 NLM
|
| 650 |
|
7 |
|a Protein Kinases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.-
|2 NLM
|
| 650 |
|
7 |
|a phosphoenolpyruvate carboxylase kinase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.1.-
|2 NLM
|
| 650 |
|
7 |
|a Protein Serine-Threonine Kinases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.11.1
|2 NLM
|
| 650 |
|
7 |
|a Type C Phospholipases
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.4.-
|2 NLM
|
| 650 |
|
7 |
|a Phosphoinositide Phospholipase C
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.4.11
|2 NLM
|
| 650 |
|
7 |
|a Phospholipase D
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.4.4
|2 NLM
|
| 650 |
|
7 |
|a Phosphoenolpyruvate Carboxylase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.1.1.31
|2 NLM
|
| 700 |
1 |
|
|a López-Baena, Francisco Javier
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Vidal, Jean
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Echevarría, Cristina
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a García-Mauriño, Sofía
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 61(2010), 10 vom: 25. Juni, Seite 2819-27
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
|
| 773 |
1 |
8 |
|g volume:61
|g year:2010
|g number:10
|g day:25
|g month:06
|g pages:2819-27
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1093/jxb/erq114
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 61
|j 2010
|e 10
|b 25
|c 06
|h 2819-27
|