One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants

*The hairpin-based RNA interference (RNAi) technique plays an important role in exploring gene function in plants. Although there are several methods for making hairpin RNA (hpRNA) constructs, these methods usually need multiple relatively laborious, time-consuming or high-cost cloning steps. Here w...

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Veröffentlicht in:The New phytologist. - 1979. - 187(2010), 1 vom: 01. Juli, Seite 240-250
1. Verfasser: Xu, Guoyong (VerfasserIn)
Weitere Verfasser: Sui, Ning, Tang, Yang, Xie, Ke, Lai, Yizhen, Liu, Yule
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins Genetic Markers RNA, Plant Green Fluorescent Proteins 147336-22-9 Glucosyltransferases EC 2.4.1.- SGT1 protein, Arabidopsis mehr... Glucuronidase EC 3.2.1.31