Characterization of hemoglobin immobilized in MgAl-layered double hydroxides by the coprecipitation method

Hemoglobin was immobilized in Mg(2)Al-Layered Double Hydroxides (LDH) by coprecipation method at pH 9.0. Interactions between Hb and LDH particles were investigated by X-ray diffraction patterns, FTIR, UV-vis, circular dichroism, and fluorescence spectroscopies. Morphology and porosity of Mg(2)Al-Hb...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 12 vom: 15. Juni, Seite 9997-10004
1. Verfasser: Charradi, Khaled (VerfasserIn)
Weitere Verfasser: Forano, Claude, Prevot, Vanessa, Madern, Dominique, Ben Haj Amara, Abdesslem, Mousty, Christine
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Hemoglobins Hydroxides Immobilized Proteins Aluminum CPD4NFA903 Magnesium I38ZP9992A
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520 |a Hemoglobin was immobilized in Mg(2)Al-Layered Double Hydroxides (LDH) by coprecipation method at pH 9.0. Interactions between Hb and LDH particles were investigated by X-ray diffraction patterns, FTIR, UV-vis, circular dichroism, and fluorescence spectroscopies. Morphology and porosity of Mg(2)Al-Hb(cop) biohybrid are analyzed from SEM and TEM images and permeability measurement. The direct electron transfer of immobilized Hb was studied by cyclic voltammetry, and the electrocatalytic activity was evaluated at glassy carbon modified with this Mg(2)Al-Hb(cop) biohybrid. Even though the percentage of electroactive Hb was less than 2%, this bioelectrode showed a low detection limit (1.5 x 10(-8) M) and a very high sensitivity (37 A/M cm(2)) for the amperometric detection of H(2)O(2) 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Hemoglobins  |2 NLM 
650 7 |a Hydroxides  |2 NLM 
650 7 |a Immobilized Proteins  |2 NLM 
650 7 |a Aluminum  |2 NLM 
650 7 |a CPD4NFA903  |2 NLM 
650 7 |a Magnesium  |2 NLM 
650 7 |a I38ZP9992A  |2 NLM 
700 1 |a Forano, Claude  |e verfasserin  |4 aut 
700 1 |a Prevot, Vanessa  |e verfasserin  |4 aut 
700 1 |a Madern, Dominique  |e verfasserin  |4 aut 
700 1 |a Ben Haj Amara, Abdesslem  |e verfasserin  |4 aut 
700 1 |a Mousty, Christine  |e verfasserin  |4 aut 
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