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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la903806a
|2 doi
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|a pubmed24n0658.xml
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|a (DE-627)NLM197275974
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|a (NLM)20384368
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Beck, Paul
|e verfasserin
|4 aut
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|a Novel type of bicellar disks from a mixture of DMPC and DMPE-DTPA with complexed lanthanides
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.07.2010
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|a Date Revised 25.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a We report on the formation of bicelles from a mixture of dimyristoylphosphatidylcholine (DMPC) and the chelator-lipid dimyristoylphosphatidylethanolamine-diethylenetriaminepentaacetate (DMPE-DTPA) with complexed lanthanides, either thulium (Tm(3+)) or lanthanum (La(3+)). The two phospholipids used have the same acyl-chain length but differ in headgroup size and chemical structure. The total lipid concentration was 15 mM, and the molar ratio of DMPC to DMPE-DTPA was 4:1. The system was studied with small angle neutron scattering (SANS) in a magnetic field, cryo-transmission electron microscopy (cryo-TEM), and (31)P NMR spectroscopy. We found that, after appropriate preparation steps, that is, extrusion through a polycarbonate membrane followed by a cooling step, monodisperse small unilamellar disks (flat cylinders called bicelles) are formed. They have a radius of 20 nm and a bilayer thickness of about 4 nm and are stable in the investigated temperature range of 2.5-30 degrees C. Fitting of SANS data with a form factor for partly aligned flat cylinders shows that the bicelles are slightly orientable in a magnetic field of 8 T if DMPE-DTPA is complexed with Tm(3+)
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|a Journal Article
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|a Lanthanoid Series Elements
|2 NLM
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|a Micelles
|2 NLM
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|a Phosphatidylethanolamines
|2 NLM
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|a Pentetic Acid
|2 NLM
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|a 7A314HQM0I
|2 NLM
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|a Dimyristoylphosphatidylcholine
|2 NLM
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|a U86ZGC74V5
|2 NLM
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|a 1,2-dimyristoylphosphatidylethanolamine
|2 NLM
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|a Z37OX1ASNK
|2 NLM
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|a Liebi, Marianne
|e verfasserin
|4 aut
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|a Kohlbrecher, Joachim
|e verfasserin
|4 aut
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|a Ishikawa, Takashi
|e verfasserin
|4 aut
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|a Rüegger, Heinz
|e verfasserin
|4 aut
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|a Fischer, Peter
|e verfasserin
|4 aut
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|a Walde, Peter
|e verfasserin
|4 aut
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|a Windhab, Erich
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 8 vom: 20. Apr., Seite 5382-7
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:26
|g year:2010
|g number:8
|g day:20
|g month:04
|g pages:5382-7
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|u http://dx.doi.org/10.1021/la903806a
|3 Volltext
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