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|a pubmed25n0657.xml
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|a (DE-627)NLM197120598
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|a (NLM)20367900
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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1 |
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|a Chen, Shan
|e verfasserin
|4 aut
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|a Induction effect of platelet-activating factor on Src-suppressed C kinase substrate gene expression in rat pulmonary microvascular endothelial cells
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 07.12.2010
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|a Date Revised 06.04.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To investigate the effect of platelet-activating factor (PAF) on the production of Src-suppressed C kinase substrate (SSeCKS) mRNA in rat pulmonary microvascular endothelial cell (RPMVEC) and its signal transduction pathways
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|a METHODS: Cellular in situ hybridization was performed to reveal changes in SSeCKS mRNA expression in the cultured RPMVECs after giving PAF stimulation
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|a RESULTS: Normal RPMVECs expressed SSeCKS mRNA at a low level, which appeared throughout the cytoplasm with specific hybridization signals. 1.5 hours of 10(-10), 10(-9), 10(-8), 10(-7) mol/L PAF incubation induced a progressive increase in SSeCKS mRNA expression. When compared to the normal control group each difference had statistical significance (0.054 9 + or - 0.000 7, 0.059 9 + or - 0.001 8, 0.069 0 + or - 0.001 8, 0.075 4 + or - 0.001 9 vs. 0.036 8 + or - 0.003 7, respectively, all P<0.05). Within 0.5, 1.5, 3, 6, 12, 24 hours of 10(-7) mol/L PAF challenge, the level of SSeCKS mRNA expression raised at 0.5 hour markedly (0.071 0 + or - 0.001 5), peaked at 1.5 hours (0.075 6 + or - 0.001 7), then began to decline gradually, and still persisted at a higher level than the normal control group until 24 hours (0.043 9 + or - 0.001 0 vs. 0.038 2 + or - 0.004 1, all P<0.05). Pre-incubation of 10 micromol/L pyrrolidine dithiocarbamate (PDTC) that inhibits activity of nuclear factor-KappaB (NF-KappaB) in RPMVECs caused a conspicuous attenuation of PAF-induced SSeCKS mRNA expression (0.049 7 + or - 0.003 2 vs. 0.071 9 + or - 0.001 9, P<0.05), whereas no change of PAF-induced effect was found by pretreatment of protein kinase C (PKC) inhibitor bis-indolylmaleimide (BIM, 0.069 7 + or - 0.002 1 vs. 0.071 9 + or - 0.001 9, P>0.05)
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|a CONCLUSION: PAF can up regulate the expression of SSeCKS mRNA in a dose- and time-dependent manner in RPMVECs. It is NF-KappaB rather than PKC signal pathway that is involved in modulation of the intracellular signaling process
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a A Kinase Anchor Proteins
|2 NLM
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|a Akap12 protein, rat
|2 NLM
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|a Cell Cycle Proteins
|2 NLM
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|a Platelet Activating Factor
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 700 |
1 |
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|a Sun, Geng-yun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a You, Qing-hai
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Nan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 22(2010), 3 vom: 06. März, Seite 135-8
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
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|g volume:22
|g year:2010
|g number:3
|g day:06
|g month:03
|g pages:135-8
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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|a GBV_ILN_350
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|a AR
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|d 22
|j 2010
|e 3
|b 06
|c 03
|h 135-8
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