A Cre-based double fluorescence indicator system for monitoring cell fusion events and selection of fused cells
We have established an in vitro Cre/loxP-based assay for monitoring cell fusion events that specifically traces the transport of cytoplasm from one cell to its fusion partner. Cells with a double fluorescence vector indicate fusion with cells expressing Cre recombinase by switching expression from r...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 48(2010), 2 vom: 01. Feb., Seite 113-20
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| 1. Verfasser: |
Pfannkuche, Kurt
(VerfasserIn) |
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| Weitere Verfasser: |
Spitkovsky, Dimitry,
Wunderlich, Frank Thomas,
Abd El Aziz, Osama Mohamed,
Saric, Tomo,
Hescheler, Jürgen,
Sachinidis, Agapios |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Luminescent Proteins
Receptors, Estrogen
Puromycin
4A6ZS6Q2CL
Cre recombinase
EC 2.7.7.-
Integrases |
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