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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la100027n
|2 doi
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|a pubmed24n0657.xml
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|a DE-627
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|a eng
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1 |
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|a Nygren, Patrik
|e verfasserin
|4 aut
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|a Secondary structure in de novo designed peptides induced by electrostatic interaction with a lipid bilayer membrane
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 02.08.2010
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|a Date Revised 18.11.2010
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|a published: Print
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|a Citation Status MEDLINE
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|a We show that it is possible to induce a defined secondary structure in de novo designed peptides upon electrostatic attachment to negatively charged lipid bilayer vesicles without partitioning of the peptides into the membrane, and that the secondary structure can be varied via small changes in the primary amino acid sequence of the peptides. The peptides have a random-coil conformation in solution, and results from far-UV circular dichroism spectroscopy demonstrate that the structure induced by the interaction with silica nanoparticles is solely alpha-helical and also strongly pH-dependent. The present study shows that negatively charged vesicles, to which the peptides are electrostatically adsorbed via cationic amino acid residues, induce either alpha-helices or beta-sheets and that the conformation is dependent on both lipid composition and variations in peptide primary structure. The pH-dependence of the vesicle-induced peptide secondary structure is weak, which correlates well with small differences in the vesicles' electrophoretic mobility, and thus the surface charge, as the pH is varied
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lipid Bilayers
|2 NLM
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|a Peptides
|2 NLM
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|a Phosphatidylglycerols
|2 NLM
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|a 1,2-dioleoyl-sn-glycero-3-phosphoglycerol
|2 NLM
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|a 66322-31-4
|2 NLM
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|a Silicon Dioxide
|2 NLM
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|a 7631-86-9
|2 NLM
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| 700 |
1 |
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|a Lundqvist, Martin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liedberg, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jonsson, Bengt-Harald
|e verfasserin
|4 aut
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| 700 |
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|a Ederth, Thomas
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 9 vom: 04. Mai, Seite 6437-48
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:26
|g year:2010
|g number:9
|g day:04
|g month:05
|g pages:6437-48
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|u http://dx.doi.org/10.1021/la100027n
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