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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2010.03217.x
|2 doi
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|a pubmed25n0656.xml
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|a (DE-627)NLM196910420
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|a (NLM)20345643
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Römer, Patrick
|e verfasserin
|4 aut
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|a Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 28.03.2011
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|a Date Revised 14.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a *Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes. *Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA). *We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes. *Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amino Acids
|2 NLM
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|a Bacterial Proteins
|2 NLM
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Glucuronidase
|2 NLM
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| 650 |
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|a EC 3.2.1.31
|2 NLM
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| 700 |
1 |
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|a Recht, Sabine
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Strauß, Tina
|e verfasserin
|4 aut
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| 700 |
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|a Elsaesser, Janett
|e verfasserin
|4 aut
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| 700 |
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|a Schornack, Sebastian
|e verfasserin
|4 aut
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| 700 |
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|a Boch, Jens
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Shiping
|e verfasserin
|4 aut
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| 700 |
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|a Lahaye, Thomas
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1990
|g 187(2010), 4 vom: 01. Sept., Seite 1048-1057
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:187
|g year:2010
|g number:4
|g day:01
|g month:09
|g pages:1048-1057
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| 856 |
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|u http://dx.doi.org/10.1111/j.1469-8137.2010.03217.x
|3 Volltext
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|d 187
|j 2010
|e 4
|b 01
|c 09
|h 1048-1057
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