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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la1003808
|2 doi
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|a pubmed24n0655.xml
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|a DE-627
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|a eng
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|a Smith, Polina B
|e verfasserin
|4 aut
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|a Investigating lyophilization of lipid nanocapsules with fluorescence correlation spectroscopy
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 31.08.2010
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|a Date Revised 20.10.2021
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|a published: Print
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|a Citation Status MEDLINE
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|a This paper describes characterization of lyophilized lipid nanocapsules loaded with Alexa 488 by fluorescence correlation spectroscopy (FCS). Fluorimetry analysis of nanocapsules containing self-quenching concentrations of 5- and 6-carboxyfluorescein was performed to establish a point of reference for FCS. FCS results complemented the results obtained by fluorimetry for a bulk nanocapsule solution and provided additional information about the size and dye retention by individual nanocapsules. Using this method, we determined that nanocapsules composed of the thiol-functionalized lipids showed the best dye retention and the most consistent results. Dye retention, size, and photolysis efficiency of these thiol-functionalized nanocapsules doped with a far-red photosensitizer did not change substantially upon lyophilization and storage at -20 degrees C for up to 2 months, making lyophilization a suitable method for the long-term storage of nanocapsules with the appropriate lipid composition
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Coloring Agents
|2 NLM
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|a Lipids
|2 NLM
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|a Nanocapsules
|2 NLM
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|a Sulfhydryl Compounds
|2 NLM
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|a Dendramis, Kimberly A
|e verfasserin
|4 aut
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|a Chiu, Daniel T
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 12 vom: 15. Juni, Seite 10218-22
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|g volume:26
|g year:2010
|g number:12
|g day:15
|g month:06
|g pages:10218-22
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|u http://dx.doi.org/10.1021/la1003808
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