The impact of biofilm growth on transport of Escherichia coli O157:H7 in sand
Copyright © 2010 The Author(s). Journal compilation © 2010 National Ground Water Association.
| Veröffentlicht in: | Ground water. - 1998. - 49(2011), 1 vom: 01. Jan., Seite 20-31 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2011
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| Zugriff auf das übergeordnete Werk: | Ground water |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Silicon Dioxide 7631-86-9 |
| Zusammenfassung: | Copyright © 2010 The Author(s). Journal compilation © 2010 National Ground Water Association. Understanding the transport behavior, survival, and persistence of pathogens such as Escherichia coli O157:H7 in the subsurface is essential to protection of public health. In this study, the transport of E. coli O157:H7 in a two-dimensional bench-scale sand aquifer system, hereafter referred to as the sandbox, was investigated, with a focus on the impact of biofilm development on E. coli retention and survival. Biofilm growth was initiated through flushing with unsterilized groundwater and addition of glucose, nitrate, and phosphate. Retention of E. coli from an injection test in clean sand, prior to promotion of biofilm growth, was approximately 9%. Subsequent to biofilm growth, 47% of injected E. coli cells were retained under similar flow conditions. After 10 d of no flow, sterile water was flushed through the biofouled sandbox and substantial concentrations (up to 1.5 × 10(5) cells/mL) of E. coli were measured in the effluent indicating that E. coli had survived the starvation period. Confocal laser scanning microscopy revealed that E. coli were located not only on the surface but also within the biofilm. Imposition of starvation conditions resulted in biofilm sloughing and possible mobilization of biofilm-associated E. coli |
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| Beschreibung: | Date Completed 05.04.2011 Date Revised 23.12.2010 published: Print Citation Status MEDLINE |
| ISSN: | 1745-6584 |
| DOI: | 10.1111/j.1745-6584.2010.00690.x |