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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erq040
|2 doi
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|a pubmed24n1340.xml
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|a (NLM)20190040
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|a eng
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|a Camps, Céline
|e verfasserin
|4 aut
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|a A transcriptomic study of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) interaction with the vascular ascomycete fungus Eutypa lata
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 01.07.2010
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|a Date Revised 22.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback; and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays comprising 14,500 probes were used to compare, under three experimental conditions (in vitro, in the greenhouse, and in the vineyard), foliar material of infected symptomatic plants (S(+)R(+)), infected asymptomatic plants (S(-)R(+)), and healthy plants (S(-)R(-)). These plants were characterized by symptom notation after natural (vineyard) or experimental (in vitro and greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative real-time PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (Erysiphe necator, Plasmopara viticola, and Botrytis cinerea). (i) Five functional categories of genes, that is those involved in metabolism, defence reactions, interaction with the environment, transport, and transcription, were up-regulated in S(+)R(+) plants compared with S(-)R(-) plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also up-regulated after infection by powdery mildew, downy mildew, and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and the polypeptide fraction secreted by Eutypa
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Kappel, Christian
|e verfasserin
|4 aut
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|a Lecomte, Pascal
|e verfasserin
|4 aut
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|a Léon, Céline
|e verfasserin
|4 aut
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|a Gomès, Eric
|e verfasserin
|4 aut
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|a Coutos-Thévenot, Pierre
|e verfasserin
|4 aut
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|a Delrot, Serge
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 61(2010), 6 vom: 15. Juni, Seite 1719-37
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:61
|g year:2010
|g number:6
|g day:15
|g month:06
|g pages:1719-37
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|u http://dx.doi.org/10.1093/jxb/erq040
|3 Volltext
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