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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la903101v
|2 doi
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|a pubmed24n0651.xml
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|a (NLM)20175580
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|a DE-627
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|e rakwb
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|a eng
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|a Wigenius, Jens A
|e verfasserin
|4 aut
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|a DNA chips with conjugated polyelectrolytes in resonance energy transfer mode
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.05.2010
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a We show how to use well-defined conjugated polyelectrolytes (CPEs) combined with surface energy patterning to fabricate DNA chips utilizing fluorescence signal amplification. Cholesterol-modified DNA strands in complex with a CPE are adsorbed to a surface energy pattern, formed by printing with soft elastomer stamps. Hybridization of the surface bound DNA strands with a short complementary strand from solution is monitored using both fluorescence microscopy and imaging surface plasmon resonance. The CPEs act as antennas, enhancing resonance energy transfer to the dye-labeled DNA when complementary hybridization of the double strand occurs
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Acetates
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|a Alexa 350
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|a Carbocyanines
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|a Chromones
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|a DNA, Single-Stranded
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|a Dimethylpolysiloxanes
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|a Electrolytes
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|a cyanine dye 5
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|a baysilon
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|a 63148-62-9
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|a Cholesterol
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|2 NLM
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|a Magnusson, Karin
|e verfasserin
|4 aut
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|a Björk, Per
|e verfasserin
|4 aut
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|a Andersson, Olof
|e verfasserin
|4 aut
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|a Inganäs, Olle
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 5 vom: 02. März, Seite 3753-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:26
|g year:2010
|g number:5
|g day:02
|g month:03
|g pages:3753-9
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|u http://dx.doi.org/10.1021/la903101v
|3 Volltext
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