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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la903232b
|2 doi
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|a pubmed25n0651.xml
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Lin, Janice
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Effect of a polymer cushion on the electrical properties and stability of surface-supported lipid bilayers
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.05.2010
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|a Date Revised 09.03.2022
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|a published: Print
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|a Citation Status MEDLINE
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|a A robust biomimetic cell membrane platform is critical for mechanistic studies of membrane protein channels. While polymer cushions are believed to facilitate the incorporation of membrane proteins in such a platform, a systematic characterization and optimization of such cushions is rarely performed. Here, we examine the influence of a polymer cushion on the electrical properties of supported 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) bilayers produced via a Langmuir-Blodgett deposition/vesicle fusion assembly process on single-crystal silicon. We show that the resistance of DPhPC bilayers is maximized at the calculated crossover concentration of the polymer (5.9 mol % PEG-lipids). Additionally, these bilayers are sufficiently stable to allow impedance analyses to be performed for nearly 3 weeks. These studies reveal the optimal PEG concentration that yields electrically robust bilayers and demonstrate the utility of the platform for future studies of membrane protein channels and membrane active peptides
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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|a Lipid Bilayers
|2 NLM
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| 650 |
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|a Polyethylene Glycols
|2 NLM
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| 650 |
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7 |
|a 3WJQ0SDW1A
|2 NLM
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| 700 |
1 |
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|a Szymanski, John
|e verfasserin
|4 aut
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1 |
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|a Searson, Peter C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hristova, Kalina
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1991
|g 26(2010), 5 vom: 02. März, Seite 3544-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:26
|g year:2010
|g number:5
|g day:02
|g month:03
|g pages:3544-8
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| 856 |
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|u http://dx.doi.org/10.1021/la903232b
|3 Volltext
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