Auxin response factor 2 (ARF2) plays a major role in regulating auxin-mediated leaf longevity

Auxin regulates a variety of physiological and developmental processes in plants. Although auxin acts as a suppressor of leaf senescence, its exact role in this respect has not been clearly defined, aside from circumstantial evidence. It was found here that ARF2 functions in the auxin-mediated contr...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 61(2010), 5 vom: 01. März, Seite 1419-30
1. Verfasser: Lim, Pyung Ok (VerfasserIn)
Weitere Verfasser: Lee, In Chul, Kim, Junyoung, Kim, Hyo Jung, Ryu, Jong Sang, Woo, Hye Ryun, Nam, Hong Gil
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't ARF2 protein, Arabidopsis Arabidopsis Proteins Indoleacetic Acids Repressor Proteins
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520 |a Auxin regulates a variety of physiological and developmental processes in plants. Although auxin acts as a suppressor of leaf senescence, its exact role in this respect has not been clearly defined, aside from circumstantial evidence. It was found here that ARF2 functions in the auxin-mediated control of Arabidopsis leaf longevity, as discovered by screening EMS mutant pools for a delayed leaf senescence phenotype. Two allelic mutations, ore14-1 and 14-2, caused a highly significant delay in all senescence parameters examined, including chlorophyll content, the photochemical efficiency of photosystem II, membrane ion leakage, and the expression of senescence-associated genes. A delay of senescence symptoms was also observed under various senescence-accelerating conditions, where detached leaves were treated with darkness, phytohormones, or oxidative stress. These results indicate that the gene defined by these mutations might be a key regulatory genetic component controlling functional leaf senescence. Map-based cloning of ORE14 revealed that it encodes ARF2, a member of the auxin response factor (ARF) protein family, which modulates early auxin-induced gene expression in plants. The ore14/arf2 mutation also conferred an increased sensitivity to exogenous auxin in hypocotyl growth inhibition, thereby demonstrating that ARF2 is a repressor of auxin signalling. Therefore, the ore14/arf2 lesion appears to cause reduced repression of auxin signalling with increased auxin sensitivity, leading to delayed senescence. Altogether, our data suggest that ARF2 positively regulates leaf senescence in Arabidopsis 
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650 7 |a Arabidopsis Proteins  |2 NLM 
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650 7 |a Repressor Proteins  |2 NLM 
700 1 |a Lee, In Chul  |e verfasserin  |4 aut 
700 1 |a Kim, Junyoung  |e verfasserin  |4 aut 
700 1 |a Kim, Hyo Jung  |e verfasserin  |4 aut 
700 1 |a Ryu, Jong Sang  |e verfasserin  |4 aut 
700 1 |a Woo, Hye Ryun  |e verfasserin  |4 aut 
700 1 |a Nam, Hong Gil  |e verfasserin  |4 aut 
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