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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2010.03192.x
|2 doi
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|a pubmed24n0651.xml
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|a (NLM)20163552
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Zhao, Fang-Jie
|e verfasserin
|4 aut
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|a The role of the rice aquaporin Lsi1 in arsenite efflux from roots
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 15.07.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a *When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated. *Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally. *Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mum arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium. *We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aquaporins
|2 NLM
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|a Arsenites
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Mercury
|2 NLM
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|a FXS1BY2PGL
|2 NLM
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|a arsenite
|2 NLM
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|a N5509X556J
|2 NLM
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1 |
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|a Ago, Yukiko
|e verfasserin
|4 aut
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|a Mitani, Namiki
|e verfasserin
|4 aut
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|a Li, Ren-Ying
|e verfasserin
|4 aut
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1 |
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|a Su, Yu-Hong
|e verfasserin
|4 aut
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|a Yamaji, Naoki
|e verfasserin
|4 aut
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|a McGrath, Steve P
|e verfasserin
|4 aut
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|a Ma, Jian Feng
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 186(2010), 2 vom: 01. Apr., Seite 392-9
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:186
|g year:2010
|g number:2
|g day:01
|g month:04
|g pages:392-9
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|u http://dx.doi.org/10.1111/j.1469-8137.2010.03192.x
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