The role of the rice aquaporin Lsi1 in arsenite efflux from roots

*When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Wh...

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Veröffentlicht in:The New phytologist. - 1979. - 186(2010), 2 vom: 01. Apr., Seite 392-9
1. Verfasser: Zhao, Fang-Jie (VerfasserIn)
Weitere Verfasser: Ago, Yukiko, Mitani, Namiki, Li, Ren-Ying, Su, Yu-Hong, Yamaji, Naoki, McGrath, Steve P, Ma, Jian Feng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Aquaporins Arsenites Plant Proteins Mercury FXS1BY2PGL arsenite N5509X556J
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520 |a *When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated. *Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally. *Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mum arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium. *We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters 
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650 7 |a arsenite  |2 NLM 
650 7 |a N5509X556J  |2 NLM 
700 1 |a Ago, Yukiko  |e verfasserin  |4 aut 
700 1 |a Mitani, Namiki  |e verfasserin  |4 aut 
700 1 |a Li, Ren-Ying  |e verfasserin  |4 aut 
700 1 |a Su, Yu-Hong  |e verfasserin  |4 aut 
700 1 |a Yamaji, Naoki  |e verfasserin  |4 aut 
700 1 |a McGrath, Steve P  |e verfasserin  |4 aut 
700 1 |a Ma, Jian Feng  |e verfasserin  |4 aut 
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