Study of the interaction of lactoferricin B with phospholipid monolayers and bilayers

Bovine lactoferricin (LfcinB) is an antimicrobial peptide obtained from the pepsin cleavage of lactoferrin. The activity of LfcinB has been extensively studied on diverse pathogens, but its mechanism of action still has to be elucidated. Because of its nonspecificity, its mode of action is assumed t...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 5 vom: 02. März, Seite 3468-78
1. Verfasser: Arseneault, Marjolaine (VerfasserIn)
Weitere Verfasser: Bédard, Sarah, Boulet-Audet, Maxime, Pézolet, Michel
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Lipid Bilayers Phosphatidylglycerols Phospholipids Water 059QF0KO0R lactoferricin B 146897-68-9 Lactoferrin mehr... EC 3.4.21.- 1,2-dipalmitoylphosphatidylglycerol VA9U6BR3SB
LEADER 01000naa a22002652 4500
001 NLM194712583
003 DE-627
005 20231223201259.0
007 cr uuu---uuuuu
008 231223s2010 xx |||||o 00| ||eng c
024 7 |a 10.1021/la903014w  |2 doi 
028 5 2 |a pubmed24n0649.xml 
035 |a (DE-627)NLM194712583 
035 |a (NLM)20112931 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Arseneault, Marjolaine  |e verfasserin  |4 aut 
245 1 0 |a Study of the interaction of lactoferricin B with phospholipid monolayers and bilayers 
264 1 |c 2010 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Completed 28.05.2010 
500 |a Date Revised 21.11.2013 
500 |a published: Print 
500 |a Citation Status MEDLINE 
520 |a Bovine lactoferricin (LfcinB) is an antimicrobial peptide obtained from the pepsin cleavage of lactoferrin. The activity of LfcinB has been extensively studied on diverse pathogens, but its mechanism of action still has to be elucidated. Because of its nonspecificity, its mode of action is assumed to be related to interactions with membranes. In this study, the interaction of LfcinB with a negatively charged monolayer of dipalmitoylphosphatidylglycerol has been investigated as a function of the surface pressure of the lipid film using in situ Brewster angle and polarization modulation infrared reflection absorption spectroscopy and on transferred monolayers by atomic force microscopy and polarized attenuated total reflection infrared spectroscopy. The data show clearly that LfcinB forms stable films at the air-water interface. They also reveal that the interaction of LfcinB with the lipid monolayer is modulated by the surface pressure. At low surface pressure, LfcinB inserts within the lipid film with its long molecular axis oriented mainly parallel to the acyl chains, while at high surface pressure, LfcinB is adsorbed under the lipid film, the hairpin being preferentially aligned parallel to the plane of the interface. The threshold for which the behavior changes is 20 mN/m. At this critical surface pressure, LfcinB interacts with the monolayer to form discoidal lipid-peptide assemblies. This structure may actually represent the mechanism of action of this peptide. The results obtained on monolayers are correlated by fluorescent probe release measurements of dye-containing vesicles made of lipids in different phases and support the important role of the lipid fluidity and packing on the activity of LfcinB 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Lipid Bilayers  |2 NLM 
650 7 |a Phosphatidylglycerols  |2 NLM 
650 7 |a Phospholipids  |2 NLM 
650 7 |a Water  |2 NLM 
650 7 |a 059QF0KO0R  |2 NLM 
650 7 |a lactoferricin B  |2 NLM 
650 7 |a 146897-68-9  |2 NLM 
650 7 |a Lactoferrin  |2 NLM 
650 7 |a EC 3.4.21.-  |2 NLM 
650 7 |a 1,2-dipalmitoylphosphatidylglycerol  |2 NLM 
650 7 |a VA9U6BR3SB  |2 NLM 
700 1 |a Bédard, Sarah  |e verfasserin  |4 aut 
700 1 |a Boulet-Audet, Maxime  |e verfasserin  |4 aut 
700 1 |a Pézolet, Michel  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Langmuir : the ACS journal of surfaces and colloids  |d 1992  |g 26(2010), 5 vom: 02. März, Seite 3468-78  |w (DE-627)NLM098181009  |x 1520-5827  |7 nnns 
773 1 8 |g volume:26  |g year:2010  |g number:5  |g day:02  |g month:03  |g pages:3468-78 
856 4 0 |u http://dx.doi.org/10.1021/la903014w  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_22 
912 |a GBV_ILN_350 
912 |a GBV_ILN_721 
951 |a AR 
952 |d 26  |j 2010  |e 5  |b 02  |c 03  |h 3468-78