Lipid transfer mediated by a recombinant pro-sterol carrier protein 2 for the accurate preparation of asymmetrical membrane vesicles requires a narrow vesicle size distribution : a free-flow electrophoresis study
We applied protein-mediated lipid transfer using recombinant His-tagged pro-sterol carrier protein 2 (pro-SCP2) to prepare asymmetrical membrane vesicles (AMV) featuring an unequal transmembrane distribution of the negative phospholipid egg-phosphatidylglycerol (EPG). Pure egg-phosphatidylcholine (E...
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