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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la903569v
|2 doi
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|a pubmed24n0648.xml
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|a eng
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|a Wendeln, Christian
|e verfasserin
|4 aut
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|a Carbohydrate microarrays by microcontact printing
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 21.06.2010
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a This Article describes the preparation of carbohydrate microarrays by the immobilization of carbohydrates via microcontact printing (microCP) on glass and silicon substrates. To this end, diene-modified carbohydrates (galactose, glucose, mannose, lactose, and maltose) were printed on maleimide-terminated self-assembled monolayers (SAMs). A Diels-Alder reaction occurred exclusively in the contact area between stamp and substrate and resulted in a carbohydrate pattern on the substrate. It was found that cyclopentadiene-functionalized carbohydrates could be printed within minutes at room temperature, whereas furan-functionalized carbohydrates required long printing times and high temperatures. By successive printing, microstructured arrays of up to three different carbohydrates could be produced. Immobilization and patterning of the carbohydrates on the surfaces was investigated with contact angle measurements, X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and fluorescence microscopy. Furthermore, the lectins concanavalin A (ConA) and peanut agglutinin (PNA) bind to the microarrays, and the printed carbohydrates retain their characteristic selectivity toward these proteins
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carbohydrates
|2 NLM
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|a Silicon
|2 NLM
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|a Z4152N8IUI
|2 NLM
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|a Heile, Andreas
|e verfasserin
|4 aut
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|a Arlinghaus, Heinrich F
|e verfasserin
|4 aut
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|a Ravoo, Bart Jan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 7 vom: 06. Apr., Seite 4933-40
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|g volume:26
|g year:2010
|g number:7
|g day:06
|g month:04
|g pages:4933-40
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|u http://dx.doi.org/10.1021/la903569v
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