Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recove...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 48(2010), 1 vom: 24. Jan., Seite 61-4
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| Auteur principal: |
Tao, Hu
(Auteur) |
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| Autres auteurs: |
Liu, Wenjun,
Simmons, Brandi N,
Harris, Helen K,
Cox, Timothy C,
Massiah, Michael A |
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| Format: | Article en ligne
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| Langue: | English |
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| Publié: |
2010
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| Accès à la collection: | BioTechniques
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| Sujets: | Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Cholic Acids
Maltose-Binding Proteins
Periplasmic Binding Proteins
Recombinant Fusion Proteins
sarkosyl
632GS99618
Octoxynol
plus...
9002-93-1
Glutathione Transferase
EC 2.5.1.18
3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate
QBP25342AG
Sarcosine
Z711V88R5F |
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