Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recove...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 48(2010), 1 vom: 24. Jan., Seite 61-4
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| 1. Verfasser: |
Tao, Hu
(VerfasserIn) |
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| Weitere Verfasser: |
Liu, Wenjun,
Simmons, Brandi N,
Harris, Helen K,
Cox, Timothy C,
Massiah, Michael A |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Cholic Acids
Maltose-Binding Proteins
Periplasmic Binding Proteins
Recombinant Fusion Proteins
sarkosyl
632GS99618
Octoxynol
mehr...
9002-93-1
Glutathione Transferase
EC 2.5.1.18
3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate
QBP25342AG
Sarcosine
Z711V88R5F |
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