Measurement of platelet aggregation in ovine blood using a new impedance aggregometer
BACKGROUND: Whole blood platelet aggregometry (impedance) is an important method to investigate platelet function disorders. Examination of hemostatic function in sheep is important with respect to their role as an animal model of human disease
| Veröffentlicht in: | Veterinary clinical pathology. - 1975. - 39(2010), 2 vom: 01. Juni, Seite 149-56 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Veterinary clinical pathology |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Fibrinolytic Agents Hirudins Receptors, Thrombin Ristocetin 1404-55-3 Arachidonic Acid 27YG812J1I Adenosine Diphosphate mehr... |
| Zusammenfassung: | BACKGROUND: Whole blood platelet aggregometry (impedance) is an important method to investigate platelet function disorders. Examination of hemostatic function in sheep is important with respect to their role as an animal model of human disease OBJECTIVE: The aim of this study was to evaluate and optimize selected methodological aspects (anticoagulant, agonist concentration) of impedance aggregometry in ovine blood using the new Multiplate 5.0 analyzer METHODS: Blood samples were collected in hirudin anticoagulant from 40 clinically healthy sheep. Samples from selected sheep were collected in citrate, with or without the addition of calcium chloride. The agonists adenosine diphosphate (ADP), collagen, ristocetin, arachidonic acid, and thrombin receptor-activating peptide (TRAP) were added in several concentrations to induce aggregation RESULTS: Based on maximum aggregation values and internal precision, no significant difference was found between ADP concentrations of 3-10 micromol/L and collagen concentrations of 3-5 microg/mL (P>.05). The lowest interindividual variation of approximately 3-4-fold was seen with 4 and 5 micromol/L ADP and 4 and 5 microg/mL collagen. Ristocetin, arachidonic acid, and TRAP did not induce significant aggregation at any concentration. Aggregation results were significantly lower when measured in citrate- vs hirudin-anticoagulated blood, regardless of the presence of calcium chloride CONCLUSIONS: Our results indicate that the multiplate impedance aggregometer is suitable for the measurement of platelet aggregation in sheep using optimal agonist concentrations of 4-5 micromol/L ADP and 4-5 microg/mL collagen. Hirudin-anticoagulated blood is the preferred sample material |
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| Beschreibung: | Date Completed 25.10.2010 Date Revised 20.10.2016 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1939-165X |
| DOI: | 10.1111/j.1939-165X.2009.00198.x |