Enzyme-mediated assimilation of DNA-functionalized single-walled carbon nanotubes

When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures w...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 2 vom: 19. Jan., Seite 613-7
1. Verfasser: Arnett, Clint M (VerfasserIn)
Weitere Verfasser: Marsh, Charles P, Welch, Charles R, Strano, Michael S, Han, Jae-Hee, Gray, Jeffry H, Carlson, Thomas A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2010
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, U.S. Gov't, Non-P.H.S. Nanotubes, Carbon DNA 9007-49-2 DNA Ligases EC 6.5.1.-
Beschreibung
Zusammenfassung:When pyrimidine-functionalized carbon nanotubes were incubated with single-stranded DNA ligase, formations of macroscopic aggregates were observed. Wet-cell transmission electron microscopy imaging revealed that the nanotubes were radially bound to form a 3D latticelike structure. These structures were not observed in control reactions lacking ligase or adenosine triphosphate. Raman spectroscopy analysis revealed no spectra indicative of carbon nanotubes in ligase-unamended controls; however, spectra were observed in radial breathing mode and in the G and G' bands in reactions containing ligase. Furthermore, the addition of deoxyribonuclease to the ligated reactions dispersed the aggregates, and a reduction in Raman spectral intensity was observed
Beschreibung:Date Completed 11.03.2010
Date Revised 13.01.2010
published: Print
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la902564n