Preliminary development and validation of a real-time reverse transcription PCR assay for the semi-quantification of viable Campylobacter jejuni in water samples

A TaqMan-based real-time reverse transcription PCR (RT-PCR) assay was developed for semi-quantification of viable Campylobacter jejuni in water samples. This preliminary assay is based on measuring the heat-shock induction of groEL messenger RNA (mRNA); the logic being that only viable cells can syn...

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Bibliographische Detailangaben
Veröffentlicht in:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 60(2009), 12 vom: 15., Seite 3151-8
1. Verfasser: Lin, S (VerfasserIn)
Weitere Verfasser: Gilpin, B, Scholes, P, Podivinsky, E, Klena, J, Savill, M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:Water science and technology : a journal of the International Association on Water Pollution Research
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Chaperonin 60 DNA, Bacterial RNA, Bacterial RNA, Messenger