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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erp343
|2 doi
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|a pubmed25n0643.xml
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|a (DE-627)NLM193014335
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|a (NLM)19933315
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Mizutani, Masanori
|e verfasserin
|4 aut
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| 245 |
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|a The syncytium-specific expression of the Orysa;KRP3 CDK inhibitor
|b implication of its involvement in the cell cycle control in the rice (Oryza sativa L.) syncytial endosperm
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 22.04.2010
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|a Date Revised 09.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a During rice (Oryza sativa L.) seed development, the primary endosperm nucleus undergoes a series of divisions without cytokinesis, producing a multinucleate cell, known as a syncytium. After several rounds of rapid nuclear proliferation, the syncytium ceases to undergo mitosis; thereafter, the syncytium is partitioned into individual cells by a specific type of cytokinesis called cellularization. The transition between syncytium and cellularization is important in determining the final seed size and is a model for studying the cell cycle and cytokinesis. The involvement of cyclin-dependent kinase (CDK) inhibitors (CKIs) in cell cycle control was investigated here during the transition between syncytium and cellularization. It was found that one of the rice CKIs, Orysa;KRP3, is strongly expressed in the caryopsis at 2 d after flowering (DAF), and its expression is significantly reduced at 3 DAF. The other CKI transcripts did not show such a shift at 2 DAF. In situ hybridization analysis revealed that Orysa;KRP3 is expressed in multinucleate syncytial endosperm at 2 DAF, but not in cellularized endosperm at 3 DAF. Two-hybrid assays showed that Orysa;KRP3 binds Orysa;CDKA;1, Orysa;CDKA;2, Orysa;CycA1;1, and Orysa;CycD2;2. By contrast, Orysa;CDKB2;1 and Orysa;CycB2;2 do not show binding to Orysa;KRP3. Orysa;KRP3 was able to rescue yeast premature cell division due to the dominant positive expression of mutant rice CDKA;1 indicating that Orysa;KRP3 inhibited rice CDK. These data suggest that Orysa;KRP3 is involved in cell cycle control of syncytial endosperm
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cyclin-Dependent Kinase Inhibitor Proteins
|2 NLM
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|a Cyclins
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Cyclin-Dependent Kinases
|2 NLM
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| 650 |
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|a EC 2.7.11.22
|2 NLM
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|a Naganuma, Takuma
|e verfasserin
|4 aut
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|a Tsutsumi, Ken-ichi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Saitoh, Yasushi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 61(2010), 3 vom: 20. März, Seite 791-8
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
1 |
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|g volume:61
|g year:2010
|g number:3
|g day:20
|g month:03
|g pages:791-8
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erp343
|3 Volltext
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|d 61
|j 2010
|e 3
|b 20
|c 03
|h 791-8
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