Antifouling surface layers for improved signal-to-noise of particle-based immunoassays

A 10-fold improvement in the signal-to-noise (S/N) ratio of an optically encoded silica particle-based immunoassay was achieved through incorporating a protein resistant poly(ethylene glycol) (PEG) surface layer and optimizing antibody immobilization conditions. PEG was activated using 2,2,2-trifluo...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1985. - 25(2009), 23 vom: 01. Dez., Seite 13510-5
1. Verfasser:	Chen, Annie (VerfasserIn)
Weitere Verfasser:	Kozak, Darby, Battersby, Bronwyn J, Forrest, Robin M, Scholler, Nathalie, Urban, Nicole, Trau, Matt
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2009
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't GPI-Linked Proteins MSLN protein, human Membrane Glycoproteins Msln protein, mouse Sulfones 2,2,2-trifluoroethanesulfonyl chloride 1648-99-3 mehr... Polyethylene Glycols 3WJQ0SDW1A Mesothelin J27WDC343N