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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1111/j.1365-2761.2009.01113.x
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Cheng, T-C
|e verfasserin
|4 aut
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|a Establishment, characterization, virus susceptibility and transfection of cell lines from cobia, Rachycentron canadum (L.), brain and fin
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|c 2010
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 05.04.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Establishment and characterization of two cobia, Rachycentron canadum, cell lines derived from cobia brain (CB) and cobia fin (CF) are described. Caudal fin and brain from juvenile cobia were dissociated for 30 and 10 min, respectively, in phosphate-buffered saline containing 0.25% trypsin at 25 degrees C. The optimal culture condition for both dissociated cells (primary cell culture) was at 28 degrees C in Leibovitz-15 medium containing 10% foetal bovine serum. The cells have been sub-cultured at a ratio of 1:2 for more than 160 passages over a period of 3 years. Origin of the cultured cells was verified by comparison of their sequences of mitochondrial cytochrome oxidase subunit I genes (cox I) with the cox 1 sequence from cobia muscle tissue. The cell lines showed polyploidy. No mycoplasma contamination was detected. Susceptibility to grouper iridovirus was observed for the CB cell line but not the CF cell line. Both cell lines expressed green fluorescent protein after being transfected with green fluorescent reporter gene driven by the cytomegalovirus promoter
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Culture Media
|2 NLM
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|a Lai, Y-S
|e verfasserin
|4 aut
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|a Lin, I-Y
|e verfasserin
|4 aut
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|a Wu, C-P
|e verfasserin
|4 aut
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|a Chang, S-L
|e verfasserin
|4 aut
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|a Chen, T-I
|e verfasserin
|4 aut
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|a Su, M-S
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 33(2010), 2 vom: 15. Feb., Seite 161-9
|w (DE-627)NLM098166034
|x 1365-2761
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| 773 |
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|g volume:33
|g year:2010
|g number:2
|g day:15
|g month:02
|g pages:161-9
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|u http://dx.doi.org/10.1111/j.1365-2761.2009.01113.x
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