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231223s2009 xx |||||o 00| ||eng c |
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|a 10.2166/wst.2009.681
|2 doi
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|e rakwb
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|a eng
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| 100 |
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|a Hernandez, M
|e verfasserin
|4 aut
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|a Addressing the role of the extrusion pump-bearing pGRT1 plasmid in toluene biodegradation by Pseudomonas putida DOT-T1E under real case scenarios
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 11.01.2010
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a The role of both the plasmid pGRT1 and the solvent extrusion pump ttgGHI during toluene biodegradation in Pseudomonas putida DOT-T1E cultures was investigated in a sterile suspended growth bioreactor operated as chemostat and inoculated independently with a wild type strain, a mutant lacking the pGRT1 plasmid (P. putida DOT-TIE-100), and a mutant with a modified pGRT1 plasmid lacking the genes encoding the ttgGHI solvent efflux pump (P. putida DOT-TIE-28). A similar process performance was recorded in all tested strains at 4 g tol m(-3) and dilution rates (D) of 0.1 h(-1). However, operation at 10 g tol m(-3) and D of 0.2 h(-1) revealed a much lower toluene EC (285 g m(-3) h(-1)) in P. putida DOT-T1E-100 cultures when compared to wild type and P. putida DOT-T1E-28 cultures (483-498 g m(-3) h(-1)), which suggests that other mechanisms rather than solvent extrusion by the ttgGHI efflux pump supported this superior process performance. Finally, the plasmid pGRT1 analysed exhibited a remarkable stability towards toluene harmful mediated effects, regardless the strain or toluene loading tested
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Carrier Proteins
|2 NLM
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|a Toluene
|2 NLM
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|a 3FPU23BG52
|2 NLM
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| 700 |
1 |
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|a Gouveia, J
|e verfasserin
|4 aut
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|a Segura, A
|e verfasserin
|4 aut
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|a Muñoz, R
|e verfasserin
|4 aut
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|a Villaverde, S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Water science and technology : a journal of the International Association on Water Pollution Research
|d 1986
|g 60(2009), 9 vom: 24., Seite 2391-8
|w (DE-627)NLM098149431
|x 0273-1223
|7 nnns
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| 773 |
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|g volume:60
|g year:2009
|g number:9
|g day:24
|g pages:2391-8
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|u http://dx.doi.org/10.2166/wst.2009.681
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