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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erp284
|2 doi
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|a pubmed24n0642.xml
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|a (DE-627)NLM192592114
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|a (NLM)19887502
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Devitt, Luke C
|e verfasserin
|4 aut
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|a Isolation and functional characterization of a lycopene beta-cyclase gene that controls fruit colour of papaya (Carica papaya L.)
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.03.2010
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|a Date Revised 20.10.2021
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|a published: Print
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|a GENBANK: FJ839871
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|a Citation Status MEDLINE
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|a The colour of papaya fruit flesh is determined largely by the presence of carotenoid pigments. Red-fleshed papaya fruit contain lycopene, whilst this pigment is absent from yellow-fleshed fruit. The conversion of lycopene (red) to beta-carotene (yellow) is catalysed by lycopene beta-cyclase. This present study describes the cloning and functional characterization of two different genes encoding lycopene beta-cyclases (lcy-beta1 and lcy-beta2) from red (Tainung) and yellow (Hybrid 1B) papaya cultivars. A mutation in the lcy-beta2 gene, which inactivates enzyme activity, controls lycopene production in fruit and is responsible for the difference in carotenoid production between red and yellow-fleshed papaya fruit. The expression level of both lcy-beta1 and lcy-beta2 genes is similar and low in leaves, but lcy-beta2 expression increases markedly in ripe fruit. Isolation of the lcy-beta2 gene from papaya, that is preferentially expressed in fruit and is correlated with fruit colour, will facilitate marker-assisted breeding for fruit colour in papaya and should create possibilities for metabolic engineering of carotenoid production in papaya fruit to alter both colour and nutritional properties
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Intramolecular Lyases
|2 NLM
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|a EC 5.5.-
|2 NLM
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|a lycopene cyclase-isomerase
|2 NLM
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|a EC 5.5.-
|2 NLM
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|a Fanning, Kent
|e verfasserin
|4 aut
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|a Dietzgen, Ralf G
|e verfasserin
|4 aut
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|a Holton, Timothy A
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 61(2010), 1 vom: 01., Seite 33-9
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:61
|g year:2010
|g number:1
|g day:01
|g pages:33-9
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|u http://dx.doi.org/10.1093/jxb/erp284
|3 Volltext
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|a AR
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|d 61
|j 2010
|e 1
|b 01
|h 33-9
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