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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la902602z
|2 doi
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|a pubmed24n0640.xml
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|a (DE-627)NLM191888508
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|a (NLM)19810705
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ratzinger, Gerda
|e verfasserin
|4 aut
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|a Surface modification of PLGA particles
|b the interplay between stabilizer, ligand size, and hydrophobic interactions
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 24.03.2010
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|a Date Revised 19.11.2015
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|a published: Print
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|a Citation Status MEDLINE
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|a Therapeutic and diagnostic carriers can be functionalized with active targeters to induce tissue-specific delivery. However, the possible impact of adsorbed steric stabilizer such as the frequently used poloxamers (Pluronics) on surface modification of poly(D,L-lactide-co-glycolide) (PLGA) particles has not been examined so far. Therefore, three model ligands of different molecular weights (653; 36,000; 155,000 g/mol) covering the size range of important targeters were conjugated to the surface of PLGA microparticles in the presence of different concentrations of Pluronic F68 (0.01-5%, w/v). Flow cytometry and fluorimetric quantification revealed for all tested ligands that high Pluronic concentrations decreased the coupling efficiency to a half or even one-third of that achieved in the absence of stabilizer. Moreover, the reduction strongly depends on the ligand size and its propensity for hydrophobic interactions. Apart from that, a high degree of particle aggregation was observed with Pluronic concentrations below 0.1% (w/v). Thus, a compromise has to be found, which combines sufficient stability with the best possible ligand coupling efficiency. For the studied system, 0.1% (w/v) turned out to be the optimum concentration of Pluronic F68
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|a Journal Article
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|a Fluorescent Dyes
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|a Immobilized Proteins
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|a Immunoglobulin G
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|a Ligands
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|a Poloxamer
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|a 106392-12-5
|2 NLM
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|a Polyglactin 910
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|a 34346-01-5
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|a Cadaverine
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|a Fluorescein
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|a TPY09G7XIR
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|a Länger, Ursula
|e verfasserin
|4 aut
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|a Neutsch, Lukas
|e verfasserin
|4 aut
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|a Pittner, Fritz
|e verfasserin
|4 aut
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|a Wirth, Michael
|e verfasserin
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|a Gabor, Franz
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 3 vom: 02. Feb., Seite 1855-9
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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|g volume:26
|g year:2010
|g number:3
|g day:02
|g month:02
|g pages:1855-9
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|u http://dx.doi.org/10.1021/la902602z
|3 Volltext
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