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231223s2010 xx |||||o 00| ||eng c |
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|a 10.1021/la902727b
|2 doi
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|a pubmed24n0639.xml
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|a (DE-627)NLM191708488
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|a (NLM)19791748
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Pastré, David
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Specific DNA-protein interactions on mica investigated by atomic force microscopy
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|c 2010
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 26.04.2010
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|a Date Revised 19.11.2015
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|a published: Print
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|a Citation Status MEDLINE
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|a DNA processing by site-specific proteins on surface remains a challenging issue for nanobioscience applications and, in particular, for high-resolution imaging by atomic force microscopy (AFM). To obtain high-resolution conditions, mica, an atomically flat and negatively charged surface, is generally used. However, even though many specific DNA/protein interactions have already been observed by AFM, little is known about DNA accessibility to specific enzymes on mica. Here we measured the accessibility of adsorbed DNA to restriction endonucleases (EcoRI and EcoRV) using AFM. By increasing the concentration of divalent or multivalent salts, DNA adsorption on mica switches from weak to strong binding. Interestingly, while the accessibility of strongly bound DNA was inhibited, loosely adsorbed DNA was efficiently cleaved on mica. This result opens new perspective to study DNA/protein interaction by AFM or to modify specifically DNA on surface
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aluminum Silicates
|2 NLM
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a Deoxyribonuclease EcoRI
|2 NLM
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| 650 |
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7 |
|a EC 3.1.21.-
|2 NLM
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|a Deoxyribonucleases, Type II Site-Specific
|2 NLM
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| 650 |
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7 |
|a EC 3.1.21.4
|2 NLM
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| 650 |
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|a GATATC-specific type II deoxyribonucleases
|2 NLM
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| 650 |
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7 |
|a EC 3.1.21.4
|2 NLM
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| 650 |
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|a mica
|2 NLM
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| 650 |
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7 |
|a V8A1AW0880
|2 NLM
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| 700 |
1 |
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|a Hamon, Loïc
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sorel, Isabelle
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Le Cam, Eric
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Curmi, Patrick A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Piétrement, Olivier
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 26(2010), 4 vom: 16. Feb., Seite 2618-23
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:26
|g year:2010
|g number:4
|g day:16
|g month:02
|g pages:2618-23
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|u http://dx.doi.org/10.1021/la902727b
|3 Volltext
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