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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2009.03028.x
|2 doi
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| 028 |
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|a pubmed24n0639.xml
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Philippe, Ryan N
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Poplar defense against insects
|b genome analysis, full-length cDNA cloning, and transcriptome and protein analysis of the poplar Kunitz-type protease inhibitor family
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|c 2009
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.05.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a *Kunitz protease inhibitors (KPIs) feature prominently in poplar defense responses against insects. The increasing availability of genomics resources enabled a comprehensive analysis of the poplar (p)KPI family. *Using genome analysis, expressed sequence tag (EST) mining and full-length (FL)cDNA cloning we established an inventory and phylogeny of pKPIs. Microarray and real-time PCR analyses were used to profile pKPI gene expression following real or simulated insect attack. Proteomics of insect midgut content was used to monitor stability of pKPI protein. *We identified 31 pKPIs in the genome and validated gene models by EST mining and cloning of 41 unique FLcDNAs. Genome organization of the pKPI family, with six poplar-specific subfamilies, suggests that tandem duplications have played a major role in its expansion. pKPIs are expressed throughout the plant and many are strongly induced by insect attack, although insect-specific signals seem initially to suppress the tree pKPI response. We found substantial peptide coverage for a potentially intact pKPI protein in insect midgut after eating poplar leaves. *These results highlight the complexity of an important defense gene family in poplar with regard to gene family size, differential constitutive and insect-induced gene expression, and resilience of at least one pKPI protein to digestion by herbivores
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|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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7 |
|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Peptides
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Protease Inhibitors
|2 NLM
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| 700 |
1 |
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|a Ralph, Steven G
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Külheim, Carsten
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jancsik, Sharon I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bohlmann, Jörg
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 184(2009), 4 vom: 01. Dez., Seite 865-84
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:184
|g year:2009
|g number:4
|g day:01
|g month:12
|g pages:865-84
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|u http://dx.doi.org/10.1111/j.1469-8137.2009.03028.x
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