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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2009.03005.x
|2 doi
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|a pubmed24n0638.xml
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|a (DE-627)NLM191421049
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|a (NLM)19761445
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Müller, Kerstin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a The NADPH-oxidase AtrbohB plays a role in Arabidopsis seed after-ripening
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.05.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a *Seeds can enter a state of dormancy, in which they do not germinate under optimal environmental conditions. Dormancy can be broken during seed after-ripening in the low-hydrated state. *By screening enhancer trap lines of Arabidopsis, we identified a role for the NADPH-oxidase AtrbohB in after-ripening. Semiquantitative PCR was used to investigate AtrbohB transcripts in seeds. These methods were complemented with a pharmacological approach using the inhibitor diphenylene iodonium chloride (DPI) and biomechanical measurements in the Brassicaceae seed model system cress (Lepidium sativum) as well as protein carbonylation assays. *atrbohB mutants fail to after-ripen and show reduced protein oxidation. AtrbohB pre-mRNA is alternatively spliced in seeds in a hormonally and developmentally regulated manner. AtrbohB is a major producer of superoxide in germinating Arabidopsis seeds, and inhibition of superoxide production by diphenylene iodonium (DPI) leads to a delay in Arabidopsis and cress seed germination and cress endosperm weakening. *Reactive oxygen species produced by AtrbohB during after-ripening could act via abscisic acid (ABA) signalling or post-translational protein modifications. Alternative splicing could be a general mechanism in after-ripening: by altered processing of stored pre-mRNAs seeds could react quickly to environmental changes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Arabidopsis Proteins
|2 NLM
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|a Onium Compounds
|2 NLM
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|a RNA, Messenger
|2 NLM
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|a Reactive Oxygen Species
|2 NLM
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|a Superoxides
|2 NLM
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|a 11062-77-4
|2 NLM
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|a diphenyleneiodonium
|2 NLM
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| 650 |
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|a 6HJ411TU98
|2 NLM
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| 650 |
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|a Abscisic Acid
|2 NLM
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| 650 |
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|a 72S9A8J5GW
|2 NLM
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| 650 |
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|a NADPH Oxidases
|2 NLM
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| 650 |
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|a EC 1.6.3.-
|2 NLM
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| 700 |
1 |
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|a Carstens, Anna Catharina
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Linkies, Ada
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Torres, Miguel Angel
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Leubner-Metzger, Gerhard
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t The New phytologist
|d 1979
|g 184(2009), 4 vom: 10. Dez., Seite 885-97
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:184
|g year:2009
|g number:4
|g day:10
|g month:12
|g pages:885-97
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|u http://dx.doi.org/10.1111/j.1469-8137.2009.03005.x
|3 Volltext
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