Semiquantitative study of the EDC/NHS activation of acid terminal groups at modified porous silicon surfaces

Infrared spectroscopy is used to investigate the transformation of carboxyl-terminated alkyl chains immobilized on a surface into succinimidyl ester-terminated chains by reaction with an aqueous solution of N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). T...

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Bibliographische Detailangaben
Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 2 vom: 19. Jan., Seite 809-14
1. Verfasser:	Sam, S (VerfasserIn)
Weitere Verfasser:	Touahir, L, Salvador Andresa, J, Allongue, P, Chazalviel, J-N, Gouget-Laemmel, A C, Henry de Villeneuve, C, Moraillon, A, Ozanam, F, Gabouze, N, Djebbar, S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2010
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Succinimides Undecylenic Acids N-hydroxysuccinimide MJE3791M4T Ethyldimethylaminopropyl Carbodiimide RJ5OZG6I4A Silicon Z4152N8IUI

Beschreibung
Zusammenfassung:	Infrared spectroscopy is used to investigate the transformation of carboxyl-terminated alkyl chains immobilized on a surface into succinimidyl ester-terminated chains by reaction with an aqueous solution of N-ethyl-N'-(3-(dimethylamino)propyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The acid chains are covalently grafted at the surface of hydrogenated porous silicon whose large specific surface area allows for assessing the activation yield in a semiquantitative way by infrared (IR) spectroscopy and detecting trace amounts of surface products and/or reaction products of small IR cross section. In this way, we rationalize the different reaction paths and optimize the reaction conditions to obtain as pure as possible succinimidyl ester-terminated surfaces. A diagram mapping the surface composition after activation was constructed by systematically varying the solution composition. Results are accounted for by NHS surface adsorption and a kinetic competition between the various EDC-induced surface reactions
Beschreibung:	Date Completed 11.03.2010 Date Revised 21.11.2013 published: Print Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la902220a