|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM190992905 |
| 003 |
DE-627 |
| 005 |
20231223190353.0 |
| 007 |
tu |
| 008 |
231223s2009 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0637.xml
|
| 035 |
|
|
|a (DE-627)NLM190992905
|
| 035 |
|
|
|a (NLM)19714891
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Skoda, M W A
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Protein density profile at the interface of water with oligo(ethylene glycol) self-assembled monolayers
|
| 264 |
|
1 |
|c 2009
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 22.09.2009
|
| 500 |
|
|
|a Date Revised 27.10.2019
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a We determined the density profile of a high-molecular-weight globular protein (bovine serum albumin, BSA) solution at the methoxy tri(ethylene glycol)-terminated undecanethiol SAM/protein solution interface by neutron reflectivity measurements. Information about the interactions between oligo(ethylene glycol) (OEG)-terminated self-assembled monolayers (SAMs) and proteins is derived from the analysis of the structure of the solid-liquid interface. The fitting results reveal oscillations of the protein density around the bulk value with decaying amplitude on a length scale of 4 to 5 nm. The amplitude, phase, period, and decay length are found to vary only slightly with temperature and the ionic strength of the protein solution. Adsorption is reversible within the limits of detection, which suggests that the hydrated ethylene glycol surface inhibits the protein from unfolding and irreversible bonding. The insensitivity of BSA adsorption toward the ionic strength of the solution contrasts with observations in surface force experiments with a fibrinogen-coated AFM tip, where electrostatic repulsion dominates theprotein/OEG SAM interaction. As reported previously, irreversible BSA adsorption takes place below 283 K, which we interpret as indicative of the presence of dynamic effects in the protein resistance of short-chain OEG-terminated surfaces
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Salts
|2 NLM
|
| 650 |
|
7 |
|a Solutions
|2 NLM
|
| 650 |
|
7 |
|a Water
|2 NLM
|
| 650 |
|
7 |
|a 059QF0KO0R
|2 NLM
|
| 650 |
|
7 |
|a Serum Albumin, Bovine
|2 NLM
|
| 650 |
|
7 |
|a 27432CM55Q
|2 NLM
|
| 650 |
|
7 |
|a Polyethylene Glycols
|2 NLM
|
| 650 |
|
7 |
|a 3WJQ0SDW1A
|2 NLM
|
| 700 |
1 |
|
|a Schreiber, F
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jacobs, R M J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Webster, J R P
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wolff, M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Dahint, R
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Schwendel, D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Grunze, M
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 7 vom: 07. Apr., Seite 4056-64
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
|
| 773 |
1 |
8 |
|g volume:25
|g year:2009
|g number:7
|g day:07
|g month:04
|g pages:4056-64
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_721
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 25
|j 2009
|e 7
|b 07
|c 04
|h 4056-64
|