Utilization of lysozyme charge ladders to examine the effects of protein surface charge distribution on binding affinity in ion exchange systems
A lysozyme library was employed to study the effects of protein surface modification on protein retention and to elucidate preferred protein binding orientations for cation exchange chromatography. Acetic anhydride was used as an acetylating agent to modify protein surface lysine residues. Partial a...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 26(2010), 2 vom: 19. Jan., Seite 759-68
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| 1. Verfasser: |
Chung, Wai Keen
(VerfasserIn) |
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| Weitere Verfasser: |
Evans, Steven T,
Freed, Alexander S,
Keba, James J,
Baer, Zachary C,
Rege, Kaushal,
Cramer, Steven M |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2010
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids
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| Schlagworte: | Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Acetic Anhydrides
acetic anhydride
2E48G1QI9Q
Muramidase
EC 3.2.1.17
Lysine
K3Z4F929H6 |
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