Nitric oxide modulates the influx of extracellular Ca2+ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes

Nitric oxide modulates the influx of extracellular Ca2+ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes

Nitric oxide (NO) plays a key role in many physiological processes in plants, including pollen tube growth. Here, effects of NO on extracellular Ca(2+) flux and microfilaments during cell wall construction in Pinus bungeana pollen tubes were investigated. Extracellular Ca(2+) influx, the intracellul...

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Veröffentlicht in:The New phytologist. - 1979. - 182(2009), 4 vom: 01. Juni, Seite 851-862
1. Verfasser: Wang, Yuhua (VerfasserIn)
Weitere Verfasser: Chen, Tong, Zhang, Chunyang, Hao, Huaiqing, Liu, Peng, Zheng, Maozhong, Baluška, František, Šamaj, Jozef, Lin, Jinxing
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2009
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Benzoates FM 4-64 Glucans Imidazoles Pyridinium Compounds Quaternary Ammonium Compounds 1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole 145757-47-7 mehr... Nitroarginine 2149-70-4 Nitric Oxide 31C4KY9ESH S-Nitroso-N-Acetylpenicillamine 79032-48-7 Pectins 89NA02M4RX callose 9064-51-1 Calcium SY7Q814VUP
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100 1 |a Wang, Yuhua  |e verfasserin  |4 aut 
245 1 0 |a Nitric oxide modulates the influx of extracellular Ca2+ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes 
264 1 |c 2009 
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500 |a Date Completed 13.06.2011 
500 |a Date Revised 29.02.2024 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a Nitric oxide (NO) plays a key role in many physiological processes in plants, including pollen tube growth. Here, effects of NO on extracellular Ca(2+) flux and microfilaments during cell wall construction in Pinus bungeana pollen tubes were investigated. Extracellular Ca(2+) influx, the intracellular Ca(2+) gradient, patterns of actin organization, vesicle trafficking and cell wall deposition upon treatment with the NO donor S-nitroso-N-acetylpenicillamine (SNAP), the NO synthase (NOS) inhibitor N(omega)-nitro-L-arginine (L-NNA) or the NO scavenger 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were analyzed. SNAP enhanced pollen tube growth in a dose-dependent manner, while L-NNA and cPTIO inhibited NO production and arrested pollen tube growth. Noninvasive detection and microinjection of a Ca(2+) indicator revealed that SNAP promoted extracellular Ca(2+) influx and increased the steepness of the tip-focused Ca(2+) gradient, while cPTIO and L-NNA had the opposite effect. Fluorescence labeling indicated that SNAP, cPTIO and L-NNA altered actin organization, which subsequently affected vesicle trafficking. Finally, the configuration and/or distribution of cell wall components such as pectins and callose were significantly altered in response to L-NNA. Fourier transform infrared (FTIR) microspectroscopy confirmed the changes in the chemical composition of walls. Our results indicate that NO affects the configuration and distribution of cell wall components in pollen tubes by altering extracellular Ca(2+) influx and F-actin organization 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Benzoates  |2 NLM 
650 7 |a FM 4-64  |2 NLM 
650 7 |a Glucans  |2 NLM 
650 7 |a Imidazoles  |2 NLM 
650 7 |a Pyridinium Compounds  |2 NLM 
650 7 |a Quaternary Ammonium Compounds  |2 NLM 
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650 7 |a Nitric Oxide  |2 NLM 
650 7 |a 31C4KY9ESH  |2 NLM 
650 7 |a S-Nitroso-N-Acetylpenicillamine  |2 NLM 
650 7 |a 79032-48-7  |2 NLM 
650 7 |a Pectins  |2 NLM 
650 7 |a 89NA02M4RX  |2 NLM 
650 7 |a callose  |2 NLM 
650 7 |a 9064-51-1  |2 NLM 
650 7 |a Calcium  |2 NLM 
650 7 |a SY7Q814VUP  |2 NLM 
700 1 |a Chen, Tong  |e verfasserin  |4 aut 
700 1 |a Zhang, Chunyang  |e verfasserin  |4 aut 
700 1 |a Hao, Huaiqing  |e verfasserin  |4 aut 
700 1 |a Liu, Peng  |e verfasserin  |4 aut 
700 1 |a Zheng, Maozhong  |e verfasserin  |4 aut 
700 1 |a Baluška, František  |e verfasserin  |4 aut 
700 1 |a Šamaj, Jozef  |e verfasserin  |4 aut 
700 1 |a Lin, Jinxing  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t The New phytologist  |d 1979  |g 182(2009), 4 vom: 01. Juni, Seite 851-862  |w (DE-627)NLM09818248X  |x 1469-8137  |7 nnns 
773 1 8 |g volume:182  |g year:2009  |g number:4  |g day:01  |g month:06  |g pages:851-862 
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