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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1021/la902247w
|2 doi
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|a pubmed24n0634.xml
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|a eng
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1 |
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|a Kang, Seoktae
|e verfasserin
|4 aut
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|a Bioinspired single bacterial cell force spectroscopy
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 03.11.2009
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|a Date Revised 21.11.2013
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|a published: Print
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|a Citation Status MEDLINE
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|a We developed a method for preparing live bacterial cell probes for atomic force microscopy (AFM) using a bioinspired polydopamine wet adhesive. Microscopic examinations with bacterial and yeast cells indicated that cells were successfully glued to the end of the AFM cantilevers and remained viable for the duration of the force measurements. Interaction forces measured with live single-cell microorganism probes differed markedly from those obtained with glutaraldehyde-fixed microorganism probes. Interaction forces between live cell probes and quartz surfaces involved both repulsive steric forces and multimodal weak adhesion forces, which were attributed to the soft exocellular polymeric layers and the heterogeneity of the cell membrane surfaces
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Polymers
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a Quartz
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|a 14808-60-7
|2 NLM
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|a Glutaral
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|a T3C89M417N
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1 |
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|a Elimelech, Menachem
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 25(2009), 17 vom: 01. Sept., Seite 9656-9
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|x 1520-5827
|7 nnns
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| 773 |
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|g volume:25
|g year:2009
|g number:17
|g day:01
|g month:09
|g pages:9656-9
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|u http://dx.doi.org/10.1021/la902247w
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