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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1002/mrc.2489
|2 doi
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|a pubmed25n0634.xml
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|a (NLM)19634131
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Neuman, Robert C
|c Jr
|e verfasserin
|4 aut
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|a Interactions of 2,2,2-trifluoroethanol with melittin
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 04.01.2010
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|a Date Revised 12.10.2009
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|a published: Print
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|a Citation Status MEDLINE
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|a 2009 John Wiley & Sons, Ltd.
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|a Melittin dissolved in 42% trifluoroethanol-water at pH 2 has been shown to be alpha-helical between residues 6 and 12 and between residues 13 and 25, with the two helical regions separated by a bend at the Leu13 residue. The inter-helix angle was found to be 154 +/- 3 degrees at 0 degrees C and 135 +/- 3 degrees at 25 degrees C. The dominant conformation of the peptide is thus similar to those observed by previous workers for the peptide in a variety of media. At 25 degrees C, intermolecular nuclear Overhauser effects arising from nuclear spin dipole-dipole interactions between melittin hydrogens and fluorines of the solvent are essentially those expected for a system that is homogeneous as regards concentration and translational diffusion of the peptide and fluoroalcohol components. However, at 0 degrees C, peptide-trifluoroethanol cross-relaxation terms are negative, a result consistent with the conclusion that fluoroalcohol molecules associate with the peptide for times (approximately 1 ns) that are long compared to the time of a typical peptide-fluoroalcohol diffusive encounter (approximately 0.2 ns). Such interactions may be responsible for the reduction of the translational diffusion coefficient of trifluoroethanol produced by dissolved peptides
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Melitten
|2 NLM
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|a 20449-79-0
|2 NLM
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|a Trifluoroethanol
|2 NLM
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|a 75-89-8
|2 NLM
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|a Gerig, John T
|e verfasserin
|4 aut
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|i Enthalten in
|t Magnetic resonance in chemistry : MRC
|d 1985
|g 47(2009), 11 vom: 01. Nov., Seite 925-31
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|g volume:47
|g year:2009
|g number:11
|g day:01
|g month:11
|g pages:925-31
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|u http://dx.doi.org/10.1002/mrc.2489
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