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231223s2009 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2009.06.011
|2 doi
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|a pubmed24n0634.xml
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|a (DE-627)NLM190198842
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|a (NLM)19628301
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Jia, Xiaoyun
|e verfasserin
|4 aut
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|a UV-B-responsive microRNAs in Populus tremula
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|c 2009
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 09.02.2010
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a MicroRNAs (miRNAs) play vital roles in down-regulating gene expression at the post-transcriptional level. A set of 24 UV-B stress-responsive miRNAs (13 up-regulated and 11 down-regulated) was identified in Populus tremula plantlet by expression profiling with our in-house miRNA filter array. Six of the UV-B-responsive miRNA and their corresponding target genes were verified for their expressions by RNA blotting and quantitative reverse transcription PCR (qRT-PCR), respectively. The predicted target genes for these miRNAs encode diverse proteins including transcription factors and phytohormone signal-related proteins. Promoter analysis of the UV-B-responsive miRNAs revealed the presence of many light-relevant cis-elements. However, these cis-elements were not necessarily specific to the promoters of UV-responsive miRNAs, indicating that other machinery may be involved in the regulation of UV-responsive miRNAs. Finally, a model was developed to describe the potential regulatory networks mediated by the UV-B-responsive miRNAs in P. tremula. These results provide new insights into the understanding of miRNAs as ubiquitous regulators in plant response to UV-B and other stresses
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a MicroRNAs
|2 NLM
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700 |
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|a Ren, Ligang
|e verfasserin
|4 aut
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|a Chen, Qi-Jun
|e verfasserin
|4 aut
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|a Li, Runzhi
|e verfasserin
|4 aut
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700 |
1 |
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|a Tang, Guiliang
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 166(2009), 18 vom: 15. Dez., Seite 2046-57
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|x 1618-1328
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|g volume:166
|g year:2009
|g number:18
|g day:15
|g month:12
|g pages:2046-57
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|u http://dx.doi.org/10.1016/j.jplph.2009.06.011
|3 Volltext
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